Enhancements and modifications of primer design program Primer3
نویسندگان
چکیده
منابع مشابه
Sequence analysis Enhancements and modifications of primer design program
Summary: The determination of annealing temperature is a critical step in PCR design. This parameter is typically derived from the melting temperature of the PCR primers, so for successful PCR work it is important to determine the melting temperature of primer accurately. We introduced several enhancements in the widely used primer design program Primer3. The improvements include a formula for ...
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ژورنال
عنوان ژورنال: Bioinformatics
سال: 2007
ISSN: 1367-4803,1460-2059
DOI: 10.1093/bioinformatics/btm091